Annexin 2 (N137) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS3553-50, BS3553-100

Sizes: 50µl, 100µl

Swiss-Prot: P07355

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: The annexin family of calcium-binding proteins is composed of at least ten mammalian genes and is characterized by a conserved core domain which binds phospholipids in a Ca2+-dependent manner and a unique amino-terminal region which may confer binding specificity. The interaction between these proteins and biological membranes has led to the hypothesis that they are involved in cellular trafficking processes such as endocytosis, exocytosis and cellular adhesion. Annexin II, also called p36, exists as a monomer or as a heterotetramer, complexed with the S-100-related protein p11. This complex is termed calpactin I. In the tetrameric form, Annexin II is an efficient substrate of PKC family and Src pp60.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Annexin 2 (N137) polyclonal antibody detects endogenous levels of Annexin 2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Annexin 2 (N137) polyclonal antibody at 1:500 dilution
Lane 1: Hela cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: Raw264.7 cell lysate
Lane 4: H9C2 cell lysate, Immunohistochemistry (IHC) analyzes of Annexin 2 (N137) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing membrane and cytoplasmic staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 38 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Annexin A2; Annexin II; Annexin-2; Calpactin I heavy chain; Calpactin-1 heavy chain; Chromobindin-8; Lipocortin II; Placental anticoagulant protein IV; PAP-IV; Protein I; p36; ANXA2; ANX2; ANX2L4; CAL1H; LPC2D; Annexin2;

Immunogen: Synthetic peptide, corresponding to amino acids 111-160 of Human Annexin 2.

Conjugate: Unconjugated

Modification: Unmodified